CRISPRDNAClustered Regularly Interspaced Short Palindromic Repeats - **Clustered.

CRISPR, . , , CRISPR.

Figure 2. General CRISPR/Cas system locus. (3) CRISPR/Cas CRISPR/Cas: AdaptationInterference. Adaptation.

Understanding the Context

CRISPR-Cas9tracrRNAcrRNAsgRNAsgRNA5DNA3tracrRNAcrRNAcrRNA sgRNA20nt.

CRISPRnonsense-mediated mRNA decay.

CRISPR 2018 CRISPR/Cas9 CCR5 .

CRISPR/Cas9 Cas9 RNAsingle guide RNA, sgRNA Cas9 RuvC HNH HNH sgRNA DNA RuvC .

Key Insights

  1. CRISPR - / .

. CRISPR-Casclustered regularly interspaced short palindrom

Cas12aCas12acrRNA DRCRISPRCRISPRdbCRISPR.